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Updated: Jul 15, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Fibronectin-adherent monocytes express tissue factor and tissue factor pathway inhibitor whereas endotoxin-stimulated

M S Bajaj1, M Ghosh, S P Bajaj

  • 1Department of Medicine, David Geffen School of Medicine, and UCLA/Orthopedic Hospital, Los Angeles, CA 90095-1690, USA. mbajaj@mednet.ucla.edu

Journal of Thrombosis and Haemostasis : JTH
|May 2, 2007
PubMed
Abstract

Insights

Fibronectin-adherent monocytes upregulate tissue factor (TF) and tissue factor pathway inhibitor (TFPI), controlling coagulation. Lipopolysaccharide-stimulated monocytes express TF but minimal TFPI, suggesting a role for TFPI in extravascular coagulation.

Area of Science:

  • Coagulation biology
  • Monocyte and immune cell function
  • Extravascular space physiology

Background:

  • Monocytes initiate coagulation via tissue factor (TF) and express TF pathway inhibitor (TFPI).
  • Lipopolysaccharide (LPS) stimulation increases monocyte TF but TFPI expression is debated.
  • The role of fibronectin-adherent monocytes in TFPI expression and TF inhibition is unclear.

Purpose of the Study:

  • To compare TF and TFPI expression in fibronectin-adherent versus LPS-stimulated non-adherent monocytes.

Main Methods:

  • Monocytes were isolated and adhered to fibronectin or stimulated with LPS.
  • TF and TFPI expression were quantified using RT-PCR, ELISA, and FX activation assays.

Main Results:

  • Fibronectin-adherent monocytes showed marked upregulation of TF (mRNA, antigen, activity) and TFPI (mRNA, antigen).
  • Fibronectin-adherent monocytes exhibited significant TFPI activity that inhibited TF procoagulant activity (PCA).
  • LPS-stimulated monocytes expressed TF but minimal TFPI, with limited inhibition of TF activity.

Conclusions:

  • Both monocyte types express TF, indicating a procoagulant phenotype.
  • Fibronectin-adherent monocytes uniquely express sufficient TFPI to regulate thrombin generation and fibrin formation.
  • TFPI expression by fibronectin-adherent monocytes is crucial for controlling coagulation in extravascular settings.

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