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Related Experiment Video

Updated: Jul 15, 2026

Breath Collection from Children for Disease Biomarker Discovery

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

An off-line breath sampling and analysis method suitable for large screening studies.

M M L Steeghs¹, S M Cristescu, P Munnik

¹Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands.

Physiological Measurement

|May 2, 2007

Summary

A new breath collection and analysis method using Tedlar bags and PTR-MS is validated for large screening studies. This method accurately measures volatile organic compounds in exhaled breath, showing good reproducibility for lung cancer detection.

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PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

Area of Science:

Analytical Chemistry
Respiratory Medicine
Biomedical Engineering

Background:

Non-invasive diagnostic methods are crucial for large-scale health screening.
Exhaled breath analysis offers a promising avenue for detecting volatile organic compounds (VOCs) associated with diseases.
Standardized and validated methods are needed for reliable breath sample collection and analysis.

Purpose of the Study:

To present and validate a new off-line breath collection and analysis method suitable for large screening studies.
To assess the accuracy, precision, selectivity, limits of detection, sensitivity, and reproducibility of the proposed method.
To demonstrate the application of the method in an exploratory case-control study for lung cancer detection.

Main Methods:

Breath collection follows American Thoracic Society guidelines for exhaled nitric oxide (NO) sampling.
Volatile gases in breath are collected in custom-made, black-layered Tedlar bags.
Collected breath samples are analyzed using proton-transfer reaction mass spectrometry (PTR-MS).

Main Results:

The method demonstrated good reproducibility, with relative standard deviations (RSD) within 12% for methanol, acetaldehyde, acetone, and water.
Isoprene content showed a slightly higher variability with 30% RSD.
An exploratory study monitored exhaled breath in 11 lung cancer patients and 57 age-matched controls ('healthy smokers').

Conclusions:

The developed off-line breath collection and PTR-MS analysis method is validated and suitable for large-scale screening studies.
The method provides reproducible measurements of key volatile organic compounds in exhaled breath.
This technique shows potential for non-invasive disease detection, as exemplified by the lung cancer case-control study.