Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shell-Isolated Nanoparticle-Enhanced Phosphorescence.

Analytical chemistry·2018
Same author

Plasmon-enhanced fluorescence spectroscopy.

Chemical Society reviews·2017
Same author

Co-Deposition of Gold Nanoparticles and Metalloporphyrin Using the Langmuir-Blodgett (LB) Technique for Surface-Enhanced Raman Scattering (SERS).

Applied spectroscopy·2015
Same author

Plasmon enhanced fluorescence with aggregated shell-isolated nanoparticles.

Analytical chemistry·2014
Same author

Raman spectroscopy and scanning electron microscopy characterizations of fission track method datable zircon grains.

Applied spectroscopy·2014
Same author

Organic acids and protein compounds causing the photoluminescence properties of natural rubber membranes and the quenching phenomena from Au nanoparticle incorporation.

Luminescence : the journal of biological and chemical luminescence·2014

Related Experiment Video

Updated: Jul 15, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Selective surface-enhanced fluorescence and dye aggregation with layer-by-layer film substrates.

David S dos Santos1, Ricardo F Aroca

  • 1University of Windsor, Department of Chemistry and Biochemistry, 401 Sunset Avenue, Windsor, Ontario, CanadaN9B 3P4.

The Analyst
|May 2, 2007
PubMed
Summary

Researchers developed selective substrates using layer-by-layer fabrication for enhanced spectroscopy. These portable substrates with metal nanoparticles offer surface enhanced fluorescence (SEF) and surface enhanced Raman scattering (SERS) for targeted ion detection.

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis
10:26

Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis

Published on: October 26, 2015

Related Experiment Videos

Last Updated: Jul 15, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis
10:26

Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis

Published on: October 26, 2015

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Spectroscopy

Background:

  • Surface-enhanced spectroscopy requires selective and target-specific enhancing substrates for advanced analytical applications.
  • Existing methods often lack the specificity needed for precise ionic species detection.

Purpose of the Study:

  • To demonstrate the concept of fabricating portable, selective substrates for surface-enhanced spectroscopy.
  • To engineer substrates capable of providing both surface-enhanced fluorescence (SEF) and surface-enhanced Raman scattering (SERS) with high selectivity.

Main Methods:

  • Utilized the layer-by-layer (LbL) technique to construct substrates incorporating metal nanoparticles.
  • Introduced metal-free polymer electrolyte top layers onto LbL substrates to impart selectivity towards ionic species.
  • Investigated the influence of surface charge on dye aggregation and the formation of J or H aggregates.

Main Results:

  • Successfully fabricated portable selective substrates using the LbL method.
  • Demonstrated that polymer electrolyte top layers confer selectivity for ionic species detection.
  • Observed that the surface charge of the top layer critically influences dye aggregation, forming J or H aggregates.

Conclusions:

  • The LbL technique is effective for creating selective enhancing substrates for surface-enhanced spectroscopy.
  • Engineered substrates show potential for targeted analytical applications using SEF and SERS.
  • Surface charge engineering of top layers offers a pathway to control dye aggregation and substrate performance.