Related Experiment Video
Updated: Jul 15, 2026
07:53
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
Published on: March 1, 2020
Development and applications of surface-activated chemical ionization
Simone Cristoni1, Simone Rubini, Luigi Rossi Bernardi
1ISB-Ion Source & Biotechnologies, Via Fantoli 16/15, 20138, Milan, Italy. simone.cristoni@gmail.com
Mass Spectrometry Reviews
|May 2, 2007
Summary
Surface-Activated Chemical Ionization (SACI) enhances analytical sensitivity by reducing chemical noise and improving ionization efficiency. This novel technique is effective for analyzing diverse biological and clinical compounds.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Biochemistry
Background:
- Traditional ionization methods can suffer from high chemical noise and limited sensitivity.
- Analyzing complex biological and clinical samples requires highly sensitive and selective techniques.
Purpose of the Study:
- To review the development and applications of Surface-Activated Chemical Ionization (SACI).
- To highlight SACI's ability to enhance sensitivity in analyzing diverse compounds.
Main Methods:
- Review of the theoretical mechanism behind SACI.
- Discussion of SACI's application in analyzing various compound classes.
Main Results:
- SACI significantly decreases chemical noise, leading to increased ionization efficiency.
- Improved sensitivity enables the analysis of compounds with varying molecular masses and polarities.
Conclusions:
- SACI represents a significant advancement in ionization source technology.
- Its benefits include enhanced sensitivity and reduced noise, making it suitable for complex sample analysis.
Related Concept Videos
01:21Chemical Ionization (CI) Mass Spectrometry
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
01:06Interfacial Electrochemical Methods: Overview
Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...
01:21Tandem Mass Spectrometry
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
01:19Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
01:09Ion-Exchange Chromatography
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
01:19Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...