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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
Published on: November 1, 2018
A 59-kD renal antigen as a new target for rapidly progressive glomerulonephritis
Vincent Audard1, Thomas Hellmark, Khalil El Karoui
1Institut de recherche en néphrologie et transplantation d'Ile de France, Henri Mondor Hospital, Paris, France. vincent.audard@hmn.ap-hop-paris.fr <vincent.audard@hmn.ap-hop-paris.fr>
Abstract:
Anti-glomerular basement membrane (anti-GBM) antibodies are the hallmark of anti-GBM disease, which is characterized by rapidly progressive glomerulonephritis. We describe the case of a 58-year-old woman who presented with rapidly progressive glomerulonephritis with typical anti-GBM staining found by means of direct immunofluorescence microscopy, associated with linear immunoglobin G deposits on tubules. Serum analysis showed circulating anti-tubular basement membrane antibodies, but failed to detect anti-GBM antibodies. Immunoblotting showed that serum antibodies reacted with a 59-kd antigen found along both the GBM and tubular basement membrane.
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