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Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag
R Lemaire1, J Stauber, M Wisztorski
1Laboratoire de Neuroimmunologie des Annélides, FRE CNRS 2933, MALDI Imaging Team, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
Abstract:
MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases.
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