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Updated: Jul 15, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
Experiments with proteins at low temperature: what do we learn on properties in their functional state?
V V Ponkratov1, J Wiedersich, J Friedrich
1Physik-Department E14, Technische Universität München, 85354 Freising, Germany.
Abstract:
The authors compared the spectral response of Zn-substituted horseradish peroxidase in a glycerol/water solvent to hydrostatic pressure at 2 K and ambient temperature. The low temperature experiments clearly demonstrate the presence of at least three different conformations with drastically different elastic properties. However, the main conformation, which determines the fluorescence spectrum at ambient temperature, did not show any significant difference between low and high temperature and pressure. The authors conclude that the local compressibility of the heme pocket of the protein depends only very weakly on temperature.
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