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Related Experiment Videos

Investigation into the subambient behavior of aqueous mannitol solutions using temperature-controlled Raman

J Renwick Beattie1, Lindsay J Barrett, John F Malone

  • 1Centre for Clinical Raman Microscopy, The Queen's University of Belfast, Belfast, United Kingdom.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V
|May 8, 2007
PubMed
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Temperature-controlled Raman microscopy (TRM) successfully located and identified mannitol forms in frozen systems. This technique revealed mannitol

Area of Science:

  • Physical Chemistry
  • Materials Science
  • Biophysics

Background:

  • Mannitol exists in various polymorphic and amorphous forms.
  • Understanding mannitol's behavior in frozen systems is crucial for pharmaceutical and food industries.
  • Crystallization and phase transitions in frozen solutions are complex phenomena.

Purpose of the Study:

  • To demonstrate temperature-controlled Raman microscopy (TRM) for locating and identifying mannitol in frozen systems.
  • To investigate the annealing behavior of mannitol solutions at -30°C.
  • To characterize different polymorphic forms of mannitol.

Main Methods:

  • Preparation and characterization of anhydrous mannitol (polymorphic forms), hemihydrate, and amorphous mannitol using X-ray diffractometry (XRD) and Raman microscopy.

Related Experiment Videos

  • Annealing of 3% w/v mannitol solutions at -30°C.
  • Mapping of frozen systems using temperature-controlled Raman microscopy (TRM) during annealing.

    • Main Results:

    • TRM successfully differentiated between various mannitol forms within frozen systems.
    • The study revealed the location of beta and delta polymorphic forms of mannitol in frozen structures for the first time.
    • Crystalline mannitol was observed to preferentially deposit at the edge of frozen drops, forming a thickening rim upon annealing.
    • Mannitol preferentially transformed to the beta form over time during annealing.
    • Spatially resolved behavior of mannitol during annealing was observed using TRM.

    Conclusions:

    • TRM is a powerful technique for studying the spatial and temporal behavior of compounds in frozen systems.
    • TRM offers significant advantages for investigating crystallization processes in frozen environments.
    • The findings provide new insights into mannitol's polymorphic transitions and distribution in frozen solutions.