Related Experiment Video
Updated: Jul 15, 2026

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Published on: November 16, 2016
Outer membrane protein H for protective immunity against Pasteurella multocida.
Jeongmin Lee1, Young Bong Kim, Moosik Kwon
1Institute of Life Science and Technology, Sungkyunkwan University, Suwon 440-746, Republic of Korea. jlee@konkuk.ac.kr
Outer membrane protein H (OmpH) from Pasteurella multocida was cloned and expressed. Antibodies against full-length OmpH provided strong protection, suggesting its potential as a vaccine candidate.
Area of Science:
- Bacteriology
- Veterinary Immunology
- Vaccine Development
Background:
- Pasteurella multocida is a significant animal pathogen causing porcine atrophic rhinitis and avian fowl cholera.
- Effective vaccines are crucial for controlling P. multocida infections in livestock.
Purpose of the Study:
- To clone and analyze the outer membrane protein H (ompH) gene from a Korean P. multocida strain.
- To evaluate the immunogenicity and protective efficacy of recombinant OmpH as a subunit vaccine candidate.
Main Methods:
- Cloning and expression of full-length and truncated ompH genes in E. coli.
- Purification of recombinant OmpH proteins.
- Immunization of mice with recombinant OmpH and subsequent challenge with P. multocida.
Main Results:
- Recombinant OmpH showed structural similarity to other serotypes.
- Recombinant OmpH proteins were antigenic and detectable with specific antisera.
- Antibodies against full-length OmpH conferred strong protection against P. multocida challenge in mice.
Conclusions:
- The full-length recombinant OmpH is a promising antigen for developing a subunit vaccine against P. multocida.
- Further research may optimize fragment-based vaccine designs for enhanced efficacy.
More Related Videos
12:53Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
Published on: March 16, 2022
13:47Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
Published on: May 19, 2020
Related Concept Videos
Multi-pass Transmembrane Proteins and β-barrels
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Antigen Processing Pathways
MHC Class I: Presenting Endogenous...
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Formation of Lipopolysaccharides