Related Experiment Video
Updated: Jul 15, 2026

Pilot In Vitro Study to Assess Cleaning Ability and Effects of Different Decontamination Methods on Implant Surfaces
Published on: November 21, 2025
Disinfection efficiency of irreversible hydrocolloid impressions using different concentrations of sodium
Maryam Memarian1, Mohamad Reza Fazeli, Hossein Jamalifar
1Department of Removable Prosthodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. memarian@tums.ac.ir
Aim:
Dental impressions are potential sources of bacterial contamination which could eventually lead to transmissible infectious diseases through the blood or saliva. Sodium hypochlorite is an effective disinfectant recommended by the American Dental Association (ADA) in a 1:10 dilution for a ten minute immersion to disinfect irreversible hydrocolloid impressions. As the ADA protocol is sometimes neglected in busy practice settings, this pilot study was designed to determine an efficient and effective protocol for disinfection of irreversible hydrocolloid impressions.
Methods And Materials:
Various concentrations of sodium hypochlorite and disinfection times were challenged against irreversible hydrocolloid impressions contaminated with six Gram-positive and Gram-negative bacteria.
Results:
A two minute immersion time in a 0.6% solution of sodium hypochlorite protocol was found to prevent bacterial growth on the impressions.
Conclusion:
Disinfection of irreversible hydrocolloid impressions in a 0.6% solution of sodium hypochlorite for two minutes was as effective as the ADA's protocol of using a 0.5% sodium hypochlorite solution for ten minutes to destroy the test bacteria.
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