Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

3.8K
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
3.8K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

1.1K
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
1.1K
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

1.5K
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
1.5K
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

2.1K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
2.1K
Development of Analytical Methods01:21

Development of Analytical Methods

2.6K
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
2.6K
Qualitative Analysis01:10

Qualitative Analysis

1.5K
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Commentary on Hoffmann E, Malolepszy L, Hochscheid C, Dettmeyer R, Fritzenwanker M. Stillbirth with a false-positive lung float test result - an unusual case report.

Forensic science, medicine, and pathology·2026
Same author

Partial cannabis legalization and the increase of the THC threshold in road traffic: a statistical analysis of traffic cases before and after legal changes.

Traffic injury prevention·2026
Same author

Commentary on: Byard RW. A forensic overview of deaths in mountainous terrain.

Forensic science, medicine, and pathology·2025
Same author

The mystery of the "air-dried chaplain" solved: the life and "afterlife" of an unusual human mummy from eighteenth century Austria.

Frontiers in medicine·2025
Same author

Postmortem mutilation in a case of natural death.

Forensic science, medicine, and pathology·2025
Same author

Sudden unexpected death due to B-cell acute lymphocytic leukaemia (B-ALL) in a 3-year-old child.

Forensic science, medicine, and pathology·2025
Same journal

A robust and validated method for the determination of 21 urinary metabolites of 15 plasticizers, including phthalates, DEHTP, and DINCH, by online SPE and liquid chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry·2026
Same journal

A label-free membrane-based biosensor array with AuNP-modified PDMS for sensitive and specific detection of alpha-fetoprotein.

Analytical and bioanalytical chemistry·2026
Same journal

Smartphone-integrated one-step colorimetric glucose detection at physiological pH enabled by a haloperoxidase mimic.

Analytical and bioanalytical chemistry·2026
Same journal

Chemiluminescence functionalized magnetic nanoparticles-based biosensor for sensitive detection of glucose, uric acid, and cholesterol.

Analytical and bioanalytical chemistry·2026
Same journal

Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.

Analytical and bioanalytical chemistry·2026
Same journal

Advancing total uronic acid quantification using a stable isotope dilution approach: validation and application to plant- and algal-derived polysaccharides.

Analytical and bioanalytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 22, 2026

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
08:54

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

Published on: May 19, 2020

8.3K

Analytical pitfalls in hair testing.

Frank Musshoff1, Burkhard Madea

  • 1Institute of Forensic Medicine, University of Bonn, Stiftsplatz 12, 53111 Bonn, Germany. f.musshoff@uni-bonn.de

Analytical and Bioanalytical Chemistry
|May 9, 2007
PubMed
Summary
This summary is machine-generated.

This review highlights potential issues in hair drug testing procedures. Understanding these pitfalls is crucial for accurate method development and interpretation of results, ensuring reliable drug analysis in hair samples.

More Related Videos

Collecting Hair Samples for Hair Cortisol Analysis in African Americans
06:01

Collecting Hair Samples for Hair Cortisol Analysis in African Americans

Published on: June 10, 2018

19.5K
Extraction and Analysis of Cortisol from Human and Monkey Hair
08:38

Extraction and Analysis of Cortisol from Human and Monkey Hair

Published on: January 24, 2014

29.2K

Related Experiment Videos

Last Updated: Feb 22, 2026

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
08:54

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

Published on: May 19, 2020

8.3K
Collecting Hair Samples for Hair Cortisol Analysis in African Americans
06:01

Collecting Hair Samples for Hair Cortisol Analysis in African Americans

Published on: June 10, 2018

19.5K
Extraction and Analysis of Cortisol from Human and Monkey Hair
08:38

Extraction and Analysis of Cortisol from Human and Monkey Hair

Published on: January 24, 2014

29.2K

Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry

Background:

  • Hair analysis offers a valuable method for long-term drug monitoring due to its stability.
  • Advances in analytical techniques have increased sensitivity and specificity for drug detection in hair.

Purpose of the Study:

  • To identify and discuss potential pitfalls in hair testing procedures.
  • To aid in the development and validation of accurate hair testing methods.
  • To prevent erroneous results and misinterpretations in drug analysis.

Main Methods:

  • Review of modern analytical procedures for drug determination in hair.
  • Critical discussion of techniques such as gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS).

Main Results:

  • Identification of common errors and challenges in hair testing.
  • Emphasis on the importance of method validation to avoid incorrect outcomes.
  • Discussion of factors influencing drug incorporation and detection in hair.

Conclusions:

  • Awareness of procedural pitfalls is essential for reliable hair drug testing.
  • Proper method development and validation are key to accurate interpretation of drug test results.
  • Continued research and critical evaluation of analytical methods are necessary.