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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Related Experiment Video

Updated: Jul 15, 2026

Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas
09:04

Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas

Published on: September 5, 2011

TRAP sequence in an acardiac twin.

Athita Chanthasenanont1, Densak Pongrojpaw

  • 1Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand. athitaka@gmail.com

Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
|May 10, 2007
PubMed
Summary

Expectant management of twin reversed-arterial perfusion (TRAP) sequence is a viable option. Close surveillance can lead to favorable outcomes for the pump twin, challenging previous mortality rates.

Area of Science:

  • Perinatology
  • Maternal-Fetal Medicine
  • Reproductive Genetics

Background:

  • Twin reversed-arterial perfusion (TRAP) sequence is a rare complication of monozygotic twinning.
  • It involves an abnormal vascular connection where one twin (acardius) develops without a heart, relying on the other twin (pump twin) for circulation.

Observation:

  • A case of TRAP sequence diagnosed sonographically at 20 weeks gestation.
  • The acardiac twin was significantly smaller and lacked defined extremities.
  • Parents opted for expectant management with serial sonographic and Doppler monitoring.

Findings:

  • The pump twin developed signs of fetal congestive heart failure (CHF) at 34 weeks.
  • Delivery via cesarean section resulted in a healthy infant.

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Model Surgical Training: Skills Acquisition in Fetoscopic Laser Photocoagulation of Monochorionic Diamniotic Twin Placenta Using Realistic Simulators
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Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye
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  • The acardius was approximately two-thirds the size of the delivered infant.
  • Implications:

    • Expectant management with close antepartum surveillance is a reasonable approach for TRAP sequence.
    • Antenatal diagnosis and monitoring may significantly reduce the previously reported neonatal mortality for the pump twin.