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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Simulated moving columns technique for enantioselective supercritical fluid chromatography
Yingru Zhang1, Jun Dai, David B Wang-Iverson
1Bioanalytical and Discovery Analytical Science, Pharmaceutical Research Institute, Bristol-Myers Squibb Company, Princeton, NJ 08648, USA. yingru.zhang@bms.com
Chirality
|May 10, 2007
Summary
Simulated Moving Columns (SMC) combined with supercritical fluid chromatography (SFC) dramatically enhances chiral separations. This SMC-SFC technique achieves high efficiency and resolution, overcoming limitations of traditional HPLC methods.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Simulated Moving Columns (SMC) is a technique for chiral separations using multiple short columns in series.
- High Performance Liquid Chromatography (HPLC) can suffer from band broadening, limiting resolution in techniques like SMC.
Purpose of the Study:
- To enhance the Simulated Moving Columns (SMC) technique by integrating it with Supercritical Fluid Chromatography (SFC).
- To demonstrate improved enantioselective separation efficiency and reduced band broadening.
Main Methods:
- Combined SMC with SFC using supercritical carbon dioxide (CO(2)) as the mobile phase.
- Utilized a multi-column system to achieve a "virtual" column length of 0.5 meters.
- Measured off-column band broadening in a two-column SMC system.
Main Results:
- SMC-SFC significantly increased the number of SMC cycles with reduced band broadening compared to SMC-HPLC.
- Achieved the first enantioselective SFC separation with enhanced efficiency.
- Off-column band broadening in SMC-SFC had a lesser impact on enantioselectivity than in SMC-HPLC.
Conclusions:
- The integration of SMC with SFC offers a powerful approach for chiral separations.
- SMC-SFC provides superior resolution and efficiency for separating enantiomers.
- This technique overcomes previous limitations associated with band broadening in chiral chromatography.
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