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Updated: Dec 27, 2025
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Effect of RSK4 on Biological Characteristics of Gastric Cancer
1The First Department of General Surgery, Zhuhai People's Hospital, Jinan University, Zhuhai City, Guangdong Province, People's Republic of China.
Purpose:
Gastric cancer is one of the most common cancers with high mortality. Emerging evidences show that ribosomal s6 kinase4 (RSK4) may be an anti-oncogene in several types of cancers, while its function in GC is still unclear. In the present study, we investigated the role of RSK4 in GC progression using MGC-803 and HGC-27 cell lines in vitro and in vivo.
Methods:
The expression of RSK4 in gastric cancer cells was evaluated using RT-qPCR and Western blot analysis. We transfected cells with RSK4 siRNA to reduce the expression of RSK4 and then evaluated the effect of RSK4 on cellular function. MTT and cell cycle assays were used to study its effect on cell growth. Flow cytometry was used to evaluate cell apoptosis. Wound healing and Transwell assays were performed to investigate metastasis. Stable cell lines with or without RSK4 knockdown were constructed with lentivirus and tumor-bearing mice were used to investigate the effect of RSK4 on cancer progression.
Results:
The results revealed that reduction of RSK4 expression inhibited cell apoptosis and promoted cell proliferation, migration, and invasion. Additionally, RSK4 knockdown promoted tumorigenesis in vivo.
Conclusion:
Our study demonstrated that RSK4 serves as a tumor suppressor in GC.
Insights
Ribosomal s6 kinase4 (RSK4) acts as a tumor suppressor in gastric cancer (GC). Reduced RSK4 expression promotes GC cell proliferation, migration, invasion, and tumorigenesis, highlighting its anti-cancer role.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Gastric cancer (GC) presents a significant global health challenge due to its high mortality rates.
- The role of ribosomal s6 kinase4 (RSK4) in GC pathogenesis remains largely undefined, despite its proposed anti-oncogenic functions in other cancers.
Purpose of the Study:
- To elucidate the functional role of RSK4 in the progression of gastric cancer.
- To investigate the impact of RSK4 expression levels on GC cell behavior in vitro and tumor development in vivo.
Main Methods:
- Quantitative real-time PCR (RT-qPCR) and Western blotting were employed to assess RSK4 expression in GC cells.
- RSK4 knockdown was achieved using siRNA and lentiviral vectors to evaluate its effects on cell proliferation, apoptosis, migration, and invasion.
- In vivo studies utilized tumor-bearing mice to assess the impact of RSK4 on tumorigenesis.
Main Results:
- Downregulation of RSK4 expression was observed to inhibit apoptosis while enhancing proliferation, migration, and invasion of GC cells.
- RSK4 knockdown significantly promoted tumor formation in vivo, indicating its suppressive role in cancer progression.
Conclusions:
- The findings strongly suggest that RSK4 functions as a tumor suppressor in gastric cancer.
- RSK4 warrants further investigation as a potential therapeutic target for gastric cancer treatment.
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