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Related Experiment Videos

mRNA expression in rabbit experimental aneurysms: a study using gene chip microarrays.

W I Mangrum1, F Farassati, R Kadirvel

  • 1Iowa Methodist Medical Center, Des Moines, Iowa, USA.

AJNR. American Journal of Neuroradiology
|May 15, 2007
PubMed
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This study characterizes molecular changes in a rabbit aneurysm model using gene chip microarrays. The findings reveal similarities to human intracranial aneurysms, particularly in metalloproteinase activity and extracellular matrix production.

Area of Science:

  • Vascular Biology
  • Molecular Medicine
  • Genomics

Background:

  • Intracranial aneurysm molecular characteristics remain poorly understood.
  • Rabbit elastase aneurysm models aid in evaluating endovascular treatment strategies.
  • Limited data exists on the molecular changes associated with aneurysm formation in animal models.

Purpose of the Study:

  • To document molecular changes in a rabbit elastase aneurysm model using gene chip microarrays.
  • To compare gene expression profiles of aneurysms with contralateral carotid arteries.
  • To identify molecular similarities between the rabbit aneurysm model and human intracranial aneurysms.

Main Methods:

  • A custom rabbit gene microarray was developed.
  • Saccular aneurysms were induced using elastase in the carotid artery of rabbits.

Related Experiment Videos

  • RNA was isolated from aneurysms and contralateral arteries for microarray analysis.
  • Reverse transcription-polymerase chain reaction (RT-PCR) was used for validation.

    • Main Results:

    • 46% of genes on the microarray showed differential expression in aneurysms compared to controls.
    • Differential gene expression indicated altered molecular pathways.
    • Rabbit aneurysms exhibited increased metalloproteinase activity and decreased extracellular matrix production, mirroring human intracranial aneurysms.
    • RT-PCR confirmed the microarray findings.

    Conclusions:

    • The molecular profile of elastase-induced saccular aneurysms in rabbits is described.
    • The rabbit aneurysm model shares key molecular features with human intracranial aneurysms.
    • The developed rabbit gene chip microarray is a valuable tool for future molecular studies of saccular aneurysms.