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Updated: Jul 15, 2026

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

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Profiling signaling polarity in chemotactic cells.

Yingchun Wang¹, Shi-Jian Ding, Wei Wang

¹Department of Pathology and Moores Cancer Center, University of California at San Diego, La Jolla, CA 92093, USA.

Proceedings of the National Academy of Sciences of the United States of America

|May 15, 2007

Summary

Cell polarization involves protein networks spatially organizing for directed movement. This study maps proteins and phosphorylation sites in the cell body versus the leading pseudopodium, revealing distinct functional networks crucial for cell migration.

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Area of Science:

Cell Biology
Proteomics
Systems Biology

Background:

Cell movement necessitates morphological polarization, including a leading pseudopodium (PD) and trailing rear.
The systemic integration of protein networks governing this polarization remains poorly understood.

Purpose of the Study:

To comprehensively map the spatial proteome and phosphoproteome of chemotactic cells.
To identify protein networks and signaling pathways localized to the cell body (CB) and PD.

Main Methods:

Global proteome profiling of CB and PD fractions.
Quantitative phosphoproteomics to analyze phosphorylation site distribution.
Comparative analysis of protein and phosphorylation site localization.

Main Results:

Spatial mapping of 3,509 proteins and 228 phosphorylation sites.
Identified distinct protein networks partitioning to PD (e.g., integrin signaling, actin regulation) and CB (e.g., DNA/RNA metabolism, cell cycle).
Revealed specific signaling proteins and phosphorylation sites enriched in either compartment.

Conclusions:

Demonstrates the spatial organization of signaling networks controlling cell polarization.
Provides a systems-level profile of proteins and phosphorylation sites critical for directed cell movement.
Offers insights into the molecular mechanisms underlying cell migration and pseudopodium formation.