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Updated: Jul 15, 2026
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
Published on: July 10, 2013
Three-dimensional cell growth on structures fabricated from ORMOCER by two-photon polymerization technique
Sabrina Schlie1, Anaclet Ngezahayo, Aleksandr Ovsianikov
1Institute of Biophysics, University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany.
Abstract:
Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER. ORMOCER related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER is shown.
