Three-dimensional cell growth on structures fabricated from ORMOCER by two-photon polymerization technique.

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Polymer Chemistry

Background:

• Three-dimensional (3D) scaffolds are crucial for tissue engineering.
• Developing biocompatible materials for scaffold fabrication is an ongoing challenge.
• ORMOCER, a hybrid organic-inorganic polymer, offers potential for 3D microfabrication.

Purpose of the Study:

• To investigate the suitability of ORMOCER for creating 3D scaffolds for tissue engineering.
• To assess the biocompatibility of ORMOCER with various cell types.
• To evaluate the impact of ORMOCER on cell proliferation, DNA integrity, and cell-to-cell adhesion.

Main Methods:

• Two-photon polymerization technique for 3D scaffold fabrication using ORMOCER.
• Cell counting and comet assay to assess cell viability and DNA damage.
• Double whole-cell patch-clamp technique to evaluate gap junction formation.
• Microscopy to observe cell growth on 3D ORMOCER structures.

Main Results:

• 3D scaffold-like structures were successfully fabricated using ORMOCER.
• ORMOCER did not affect the doubling time or cause DNA strand breaks in CHO, GFSHR-17, GM-7373, and SH-SY5Y cells.
• No alterations in gap junction formation were observed, indicating preserved cell-to-cell adhesion.
• Cells demonstrated growth on the vertical surfaces of the 3D ORMOCER structures.

Conclusions:

• ORMOCER is a promising biocompatible material for fabricating 3D scaffolds in tissue engineering.
• The material supports cell growth and maintains cell integrity and function.
• ORMOCER scaffolds are suitable for applications requiring cell adhesion and proliferation.