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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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25,000-fold pre-concentration in a single step with liquid-phase microextraction.

Tung Si Ho1, Terje Vasskog, Trude Anderssen

  • 1School of Pharmacy, University of Oslo, Oslo, Norway.

Analytica Chimica Acta
|May 15, 2007
PubMed
Summary

A new hollow fiber protected liquid-phase microextraction (LPME) method efficiently extracts antidepressant drugs from large water volumes. This technique achieves high enrichment and sensitive detection in environmental water samples.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Liquid-phase microextraction (LPME) is a valuable technique for sample preparation.
  • Extracting analytes from large sample volumes presents challenges for traditional LPME methods.
  • Developing efficient methods for detecting pharmaceuticals in environmental matrices is crucial.

Purpose of the Study:

  • To develop and optimize a hollow fiber protected liquid-phase microextraction (LPME) method for large-volume water samples.
  • To investigate the extraction efficiency of antidepressant drugs from environmental water.
  • To enable sensitive detection of these drugs using HPLC-UV and HPLC-MS.

Main Methods:

  • Hollow fiber protected liquid-phase microextraction (LPME) using dihexyl ether as the liquid membrane.
  • Extraction of five antidepressant drugs from 100 or 1100 mL water samples at pH 11.8.
  • Analysis of acceptor solutions via High-Performance Liquid Chromatography with Ultraviolet (HPLC-UV) or Mass Spectrometry (HPLC-MS) detection.

Main Results:

  • Recoveries of 33-49% and enrichments of 18,000-27,000 were achieved from 1100 mL samples after 120 min.
  • Detection limits down to 5-30 pg L(-1) were obtained using HPLC-MS.
  • Good linearity (r2 > 0.982) was observed in the 1-500 ng L(-1) range, with within-day precision < 20.6% R.S.D.

Conclusions:

  • The developed hollow fiber protected LPME method is effective for large-volume water sample extraction.
  • The method allows for sensitive and reliable quantification of antidepressant drugs in environmental waters.
  • This technique offers a promising approach for monitoring pharmaceutical pollution in aquatic environments.