Related Experiment Video
Updated: Jul 15, 2026

Determination of the Absorption, Translocation, and Distribution of Imidacloprid in Wheat
Published on: April 28, 2023
Direct determination of glyphosate using hydrophilic interaction chromatography with coulometric detection at copper
Cláudia F B Coutinho1, Lincoln F M Coutinho, Luiz H Mazo
1Universidade de São Paulo, Instituto de Química de São Carlos, Av. Trabalhador São-carlense 400, Caixa Postal 780, CEP 13566-590 São Carlos, SP, Brazil. claudiabreda@yahoo.com.br
Abstract:
A simple, rapid, and low-cost coulometric method for direct detection of glyphosate using hydrophilic interaction chromatography is presented. The principle of detection is based on the enhancement of the anodic current of copper microelectrode in the presence of complexing agents, such as glyphosate, with the formation of a soluble Cu(II) complex. Under optimized conditions, the limit of detection (S/R=3) for glyphosate was 0.1 mg L(-1) (0.59 microM) without any preconcentration method. The calibration curve has been found linear in all concentration range tested (from limit of detection to 34 mg L(-1)) with an excellent correlation coefficient (0.9999). The present method was successfully applied for the determination of glyphosate in fruit juices without any kind of extraction, clean-up, or preconcentration step, with recoveries of 92 and 90% for apple and grape juice, respectively.
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Detectors-II
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Controlled-Current Coulometry: Overview
Controlled-Potential Coulometry: Electrolytic Methods
The chosen potential ensures...
