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Reevaluating the capability of Taq DNA polymerase: long PCR amplification
Hayan Lee1, Kyung-Nam Kim, Young Kee Chae
1Department of Chemistry, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul, Korea.
Protein and Peptide Letters
|May 17, 2007
Abstract:
We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the "long PCR" up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a "long buffer." We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5' to 3' exonuclease activity of Taq.
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