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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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SERS at structured palladium and platinum surfaces.

Mamdouh E Abdelsalam1, Sumeet Mahajan, Philip N Bartlett

  • 1School of Chemistry, University of Southampton, Southampton, SO17 1BJ, UK.

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|May 18, 2007
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Summary

Researchers developed novel palladium and platinum films using templated electrodeposition. These structured metal surfaces enable stable, reproducible surface-enhanced Raman spectroscopy (SERS) for studying adsorbed molecules.

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Area of Science:

  • Materials Science
  • Surface Chemistry
  • Spectroscopy

Background:

  • Palladium and platinum are crucial catalytic metals.
  • Studying reactive species on metal surfaces is vital for catalysis.
  • Surface-enhanced Raman spectroscopy (SERS) offers potential for surface analysis.

Purpose of the Study:

  • To create thin palladium and platinum films with ordered void structures.
  • To evaluate these structured films as substrates for SERS.
  • To demonstrate the utility of these SERS substrates for studying adsorbed molecules.

Main Methods:

  • Templated electrodeposition using colloidal templates to create structured metal films.
  • Fabrication of palladium and platinum films with hexagonal arrays of sphere segment voids.
  • Acquisition of SERS spectra for adsorbed benzenethiol on structured metal surfaces.

Main Results:

  • Achieved stable and reproducible SERS enhancements from non-rough metal films.
  • Demonstrated SERS signal enhancement factors of 1800 for palladium and 550 for platinum at 633 nm.
  • Correlated SERS enhancement with film thickness and void diameter.

Conclusions:

  • Templated electrodeposition yields effective SERS substrates from palladium and platinum.
  • Structured metal films provide a platform for studying adsorbed species via SERS.
  • Optimized film parameters are key to achieving significant SERS enhancement.