Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PDNA as building blocks for membrane-guided self-assemblies.

D Pompon1, A Laisné

  • 1Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, UPR2167, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France. pompon@cgm.cnrs-gif.fr

Biochemical Society Transactions
|May 22, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential azole antifungal efficacies contrasted using a Saccharomyces cerevisiae strain humanized for sterol 14 alpha-demethylase at the homologous locus.

Antimicrobial agents and chemotherapy·2008
Same author

Unique supramolecular assembly of a redox protein with nucleic acids onto hybrid bilayer: towards a dynamic DNA chip.

Biosensors & bioelectronics·2005
Same author

The use of Yarrowia lipolytica for the expression of human cytochrome P450 CYP1A1.

Yeast (Chichester, England)·2004
Same author

Microarray-based method for combinatorial library sequence mapping and characterization.

BioTechniques·2003
Same author

Human epoxide hydrolase is the target of germander autoantibodies on the surface of human hepatocytes: enzymatic implications.

Advances in experimental medicine and biology·2002
Same author

Design and characterization of a novel "family-shuffling" technology adapted to membrane enzyme: application to P450s involved in xenobiotic metabolism.

Advances in experimental medicine and biology·2002
Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026
Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026
Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026
Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026
Same journal

Implications of Rho GTPase signaling in cancer immunotherapy.

Biochemical Society transactions·2026
See all related articles

Researchers created novel protein-DNA complexes (PDNAs) for self-assembling nanostructures. These structures allow precise control over the arrangement of redox and absorbing centers for advanced materials.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Synthetic Biology

Background:

  • Protein-DNA complexes (PDNAs) offer unique properties for constructing advanced nanomaterials.
  • Developing controllable methods for assembling these complexes is crucial for creating functional nanostructures.

Purpose of the Study:

  • To design and synthesize novel semi-synthetic PDNAs with tuneable properties.
  • To develop a controllable solid-phase synthesis strategy for polymerizing these PDNAs into ordered nanostructures.
  • To characterize the self-assembly kinetics and resulting nano-object structures.

Main Methods:

  • Design and synthesis of semi-synthetic PDNAs incorporating a cytochrome b(5) scaffold, redox cofactor, linker, and oligonucleotide.
  • Attachment of PDNA building blocks to Ni-DOGS-doped supported membranes via metal chelate bridges.

Related Experiment Videos

  • Solid-phase polymerization using stepwise addition of complementary oligonucleotides.
  • Kinetic analysis using surface plasmon resonance imagery (SPRI).
  • Structural characterization via gel electrophoresis and single-molecule approaches with quantum dot labeling.

    • Main Results:

    • Successfully designed, synthesized, and purified homogeneous semi-synthetic PDNAs.
    • Demonstrated controllable polymerization of PDNAs on supported membranes, creating regular patterns of redox and absorbing centers.
    • Achieved tuneable distances and geometry of patterned centers.
    • Determined self-assembly kinetics and characterized the nano-object structures.

    Conclusions:

    • The developed PDNA building blocks and solid-phase synthesis strategy enable the creation of precisely patterned nanostructures.
    • These self-assembled nano-objects offer a versatile platform for applications requiring controlled arrangement of functional centers.