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Related Experiment Videos

Microencapsulated hepatocytes. Prospects for extracorporeal liver support.

H W Matthew1, S O Salley, W D Peterson

  • 1Department of Chemical Engineering, Wayne State University, Detroit, Michigan 48202.

ASAIO Transactions
|July 11, 1991
PubMed
Summary

Encapsulated hepatocytes show promise for bioartificial liver support. Perfusion bioreactors significantly enhanced urea synthesis compared to plate cultures, indicating improved liver function in this system.

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Area of Science:

  • Biotechnology
  • Hepatology
  • Biomedical Engineering

Background:

  • Bioartificial liver support systems aim to restore liver function.
  • Encapsulated hepatocytes offer a potential solution for liver support.
  • Complex coacervation is a technique for cell encapsulation.

Purpose of the Study:

  • To evaluate encapsulated rabbit hepatocytes in a bioartificial liver support system.
  • To compare the efficacy of hepatocytes cultured in perfusion reactors versus plate cultures.
  • To assess urea synthesis and drug metabolism capabilities.

Main Methods:

  • Rabbit hepatocytes were encapsulated using complex coacervation.
  • Hepatocytes were cultured in multicomponent capsules on plates and in a perfusion reactor.

Related Experiment Videos

  • Urea synthesis, antipyrine, and diazepam degradation rates were measured over 10 days.

    • Main Results:

    • Urea synthesis rates were significantly higher in perfusion cultures compared to plate cultures.
    • Drug degradation rates (antipyrine and diazepam) did not differ significantly across culture systems.
    • Encapsulated hepatocytes maintained function for up to 10 days.

    Conclusions:

    • Perfusion bioreactors enhance urea synthesis of encapsulated hepatocytes, suggesting improved metabolic function.
    • Encapsulated hepatocytes show potential for bioartificial liver support, particularly in perfusion systems.
    • Further research is needed to optimize drug metabolism in encapsulated hepatocyte systems.