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Updated: Jul 14, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain
Karen Orjuela1, Catalina Amador, Manuel A Patarroyo
1Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Bogota, Colombia.
Abstract:
Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.
