Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Useful 'junk': Alu RNAs in the human transcriptome.

J Häsler1, T Samuelsson, K Strub

  • 1Université de Genève, Département de Biologie Cellulaire, 30 quai Ernest Ansermet, 1211 Genève-4, Switzerland.

Cellular and Molecular Life Sciences : CMLS
|May 22, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Total and unicondylar knee arthroplasty are equivalent treatment options in end-stage spontaneous osteonecrosis of the knee, and the size of the lesion has no influence on the results.

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2020
Same author

Direct binding of the Alu binding protein dimer SRP9/14 to 40S ribosomal subunits promotes stress granule formation and is regulated by Alu RNA.

Nucleic acids research·2014
Same author

Removal of Small-Sized Kidney Stones Fragments Using Inverted Flushing Procedure in Ureteroscopy.

Biomedizinische Technik. Biomedical engineering·2013
Same author

[Conclusions and recommendations of a WHO expert consultation meeting on iron supplementation for infants and young children in malaria endemic areas].

Medecine tropicale : revue du Corps de sante colonial·2008
Same author

Glomerulus-specific mRNA transcripts and proteins identified through kidney expressed sequence tag database analysis.

Kidney international·2007
Same author

Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same journal

PHLDA1 knockdown exacerbates emphysema in COPD by attenuating proliferation of alveolar type II cells via modulating GLI1 nuclear translocation.

Cellular and molecular life sciences : CMLS·2026
Same journal

Single-Cell dissection of fibrodysplasia ossificans progressiva identifies SPP1 as a mediator of macrophage-fibroadipogenic progenitors crosstalk.

Cellular and molecular life sciences : CMLS·2026
Same journal

Ablation of the renal tubular gluconeogenic enzyme PCK1 drives AKI-to-CKD transition by negatively regulating the TGF-β/Smad3 signaling pathway.

Cellular and molecular life sciences : CMLS·2026
Same journal

LncRNA modulates Dpp-mediated wing development to influence flight in Aedes aegypti.

Cellular and molecular life sciences : CMLS·2026
Same journal

TROP2 promotes bone metastasis of colorectal cancer through interaction with the fibronectin-integrin axis.

Cellular and molecular life sciences : CMLS·2026
Same journal

PRMT5-Cacna1d axis maintains calcium homeostasis to regulate postnatal motor development in mice.

Cellular and molecular life sciences : CMLS·2026
See all related articles

Alu elements, once considered junk DNA, are abundant in the human genome. These repetitive elements, transcribed as free or embedded Alu RNAs, significantly regulate gene expression post-transcriptionally.

Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • Alu elements are the most abundant repetitive sequences in the human genome, with over one million copies.
  • These elements have amplified through retrotransposition.
  • Alu elements can be transcribed by RNA Polymerase III (Pol III) as 'free Alu RNAs' or by RNA Polymerase II (Pol II) as 'embedded Alu RNAs'.

Purpose of the Study:

  • To investigate the functional roles of free and embedded Alu RNAs in gene expression regulation.
  • To understand how sequences previously classified as 'junk DNA' contribute to cellular functions.

Main Methods:

  • Analysis of Alu RNA transcription by Pol III and Pol II.
  • Investigation of the impact of Alu RNAs on post-transcriptional gene regulation mechanisms.

Related Experiment Videos

  • Experimental validation of Alu RNA functions in controlling gene expression.

    • Main Results:

    • Both free and embedded Alu RNAs are actively involved in post-transcriptional gene regulation.
    • Alu RNAs influence key processes including protein translation, alternative splicing, and mRNA stability.
    • Demonstration of functional roles for previously disregarded genomic elements.

    Conclusions:

    • Alu RNAs are crucial regulators of gene expression, highlighting a functional role for repetitive DNA.
    • The human genome's 'junk DNA' harbors regulatory elements essential for cellular function.
    • These findings redefine our understanding of genomic regulation and the utility of repetitive elements.