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Spectrophotometry: Introduction

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Related Experiment Video

Updated: Jul 14, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

[A new measurement method of time-resolved spectrum].

Zhi-gang Shi¹, Shi-hua Huang, Chun-jun Liang

¹Institute of Optoelectronic Technology, Beijing Jiaotong University, Beijing 100044, China. zg_shi@126.com

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|May 23, 2007

Summary

A novel method enables time-resolved spectrum (TRS) measurement using a micro-controller and monochromator. This technique accurately captures spectral data over time, proving feasible and convenient for material analysis.

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A Multimodal Wide-Field Fourier-Transform Raman Microscope

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Last Updated: Jul 14, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

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A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

Spectroscopy
Materials Science
Analytical Chemistry

Context:

Accurate characterization of material properties requires advanced spectroscopic techniques.
Traditional methods for time-resolved spectrum (TRS) measurement can be complex and time-consuming.
Developing efficient and reliable spectroscopic tools is crucial for scientific advancement.

Purpose:

To introduce a new, feasible, credible, and convenient method for measuring time-resolved spectrum (TRS).
To detail the hardware and software components enabling automated spectral acquisition.
To demonstrate the method's application in analyzing Tb3+ luminescence.

Summary:

A novel time-resolved spectrum (TRS) measurement method is presented, utilizing an AT89C51 micro-controller to drive a monochromator and a photomultiplier tube for signal detection.
Data acquisition involves an oscillograph and RS232 serial interfaces for computer transmission, enabling the generation of attenuation curves and TRS.
The method performs parallel measurements in the time scale and serial measurements in the wavelength scale, successfully characterizing Tb(o-BBA)3 phen, including its 3D fluorescence intensity-wavelength-time spectra.

Impact:

This method provides a practical approach for obtaining time-resolved spectra and integrated emission spectra.
It facilitates the detailed analysis of luminescent materials like Tb(o-BBA)3 phen.
The validated technique offers a convenient and reliable tool for researchers in spectroscopy and materials science.