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Phospholipid acylation by mouse sciatic nerve microsomes.

C Cassagne1, H Juguelin, F Boiron

  • 1Institut de Biochimie Cellulaire et Neurochimie du CNRS, Université de Bordeaux II, France.

Biochimica Et Biophysica Acta
|November 18, 1991
PubMed
Summary
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Mouse sciatic nerve microsomes selectively transfer oleoyl-CoA to lysolipid acceptors, forming phospholipids. This process is independent of lysophospholipids and occurs at high membrane concentrations.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Cell Biology

Background:

  • Acyl-CoA esters are crucial lipid precursors.
  • Lysophospholipids are key intermediates in membrane lipid metabolism.
  • Sciatic nerve myelin sheath relies on specific lipid synthesis pathways.

Purpose of the Study:

  • To investigate the partitioning and transfer of oleoyl-CoA in mouse sciatic nerve microsomes.
  • To identify the preferred lysolipid acceptors for acyl transfer.
  • To understand the formation of phospholipids like phosphatidylcholine (PC).

Main Methods:

  • Incubation of mouse sciatic nerve microsomes with [1-14C]oleoyl-CoA.
  • Analysis of oleoyl-CoA partitioning in the presence and absence of lysophospholipids.
  • Quantification of acyl transfer to various exogenous acyl acceptors.

Related Experiment Videos

  • Detection of de novo phospholipid formation.

    • Main Results:

    • Oleoyl-CoA partitioning (45%) was unaffected by lysophospholipids.
    • Selective acyl transfer occurred, with oleoyl > myristoyl, palmitoyl, stearoyl.
    • Acylglycerophosphocholine and acylglycerophosphoinositol were preferred acceptors.
    • Phosphatidylcholine formation was observed from acylGPC and oleoyl-CoA.

    Conclusions:

    • Mouse sciatic nerve microsomes exhibit selective acyl transfer from oleoyl-CoA to specific lysolipid acceptors.
    • This acyl transfer is important for phospholipid synthesis in nerve membranes.
    • The findings contribute to understanding lipid metabolism in myelinated axons.