Related Experiment Video
Updated: Jul 14, 2026
06:01
Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
Published on: October 6, 2022
Beta1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in
Deborah K Lieu1, Lea A Degraffenried, Roslyn R Isseroff
1Departments of Urology, University of California at Davis, Sacramento, California 95817, USA.
Tissue Engineering
|May 24, 2007
Summary
Researchers identified beta(1) integrin as a marker to isolate urothelial stem cells. Flow cytometry enrichment yielded highly clonogenic and proliferative cells, unlike rapid adhesion methods.
Area of Science:
- Urology
- Stem Cell Biology
- Tissue Engineering
Background:
- Isolating tissue-specific stem cells is challenging due to a lack of distinct markers.
- Beta(1) integrin has been used to enrich for epithelial stem cells in other tissues.
- Urothelial stem cells are crucial for bladder tissue engineering and gene therapy.
Purpose of the Study:
- To report the first successful enrichment of putative urothelial stem cells.
- To compare the efficacy of rapid adhesion and flow cytometry methods for urothelial stem cell isolation.
- To evaluate the clonogenic and proliferative potential of enriched urothelial cells.
Main Methods:
- Utilized beta(1) integrin expression for cell enrichment.
- Employed rapid adhesion to collagen IV and flow cytometry techniques.
- Assessed cell function through clonogenic and proliferative assays.
Main Results:
- Flow cytometry enrichment based on beta(1) integrin yielded highly clonogenic and proliferative urothelial cells.
- Rapid adhesion method was less efficient for urothelial stem cell isolation.
- Beta(1) integrin is a viable marker for enriching functional urothelial stem cells.
Conclusions:
- Flow cytometry using beta(1) integrin is an effective method for isolating functional urothelial stem cells.
- The unique nature of transitional epithelium may explain the lower efficiency of the rapid adhesion method.
- This study provides a foundation for advancing urothelial tissue engineering and gene therapy applications.
Related Concept Videos
01:10Integrins
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...