Heparanase expression in gallbladder carcinoma and its correlation to prognosis
Wujun Wu1, Cheng'en Pan, Haibo Yu
1The First Affiliated Hospital of Xi'an Jiaotong University, Department of Hepatobiliary surgery, Jiankang Road, Xi'an, China. wuwujun_xa@163.com
Journal of Gastroenterology and Hepatology
|May 26, 2007
Summary
Heparanase and hypoxia-inducible factor-1alpha (HIF-1alpha) are frequently found in gallbladder cancer and linked to poorer survival. High heparanase, with nuclear transcriptional factor kappa B p65 (NF-kappaB p65), may drive gallbladder cancer invasion and metastasis.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Heparanase, an enzyme cleaving heparan sulfate, is implicated in tumor growth and spread.
- Understanding heparanase and key transcription factors' roles is crucial for gallbladder cancer prognosis.
Purpose of the Study:
- To analyze heparanase, hypoxia-inducible factor-1alpha (HIF-1alpha), and nuclear transcriptional factor kappa B p65 (NF-kappaB p65) expression in gallbladder cancer.
- To explore their prognostic value in gallbladder carcinoma patients.
Main Methods:
- Immunohistochemistry was used to detect protein levels of heparanase, HIF-1alpha, and NF-kappaB p65.
- Analysis of 38 gallbladder carcinoma specimens for clinicopathological significance.
Main Results:
- Heparanase, HIF-1alpha, and NF-kappaB p65 were detected in 63.2%, 34.2%, and 57.9% of specimens, respectively.
- High heparanase expression correlated with advanced TNM stage, deeper invasion, lymph node metastasis, and reduced 3-year survival.
- HIF-1alpha and NF-kappaB p65 correlated with tumor size; HIF-1alpha positivity was linked to decreased survival. Heparanase-positive cases showed higher NF-kappaB p65 expression.
Conclusions:
- Heparanase and HIF-1alpha are frequently expressed in gallbladder carcinoma and associated with decreased survival.
- Combined high expression of heparanase and NF-kappaB p65 may contribute to gallbladder cancer's invasive and metastatic potential.