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Updated: Jul 14, 2026
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Emerging methods in proteomics: top-down protein characterization by multistage tandem mass spectrometry
Gwynyth Scherperel1, Gavin E Reid
1Department of Chemistry, Michigan State University, 234 Chemistry Building, East Lansing, MI 48824, USA.
The Analyst
|May 26, 2007
Summary
Top-down mass spectrometry offers a powerful way to analyze protein modifications. This review covers current strategies, challenges, and solutions for top-down protein characterization using mass spectrometry.
Area of Science:
- Proteomics
- Analytical Chemistry
- Biochemistry
Background:
- Conventional bottom-up mass spectrometry has limitations for comprehensive protein analysis.
- Top-down mass spectrometry (TDMS) provides an alternative for intact protein characterization.
- Protein modifications are crucial for biological function and disease.
Purpose of the Study:
- To provide an overview of current top-down mass spectrometry strategies.
- To discuss technical challenges in TDMS implementation.
- To present solutions for TDMS on various mass spectrometry platforms.
Main Methods:
- Review of existing literature on top-down mass spectrometry techniques.
- Analysis of strategies for intact protein analysis.
- Discussion of mass spectrometry instrumentation for TDMS.
Main Results:
- Top-down approaches are effective for characterizing co- and post-translational modifications.
- Key technical challenges in TDMS include data acquisition and analysis.
- Solutions exist for implementing TDMS across different mass spectrometry platforms.
Conclusions:
- Top-down mass spectrometry is a valuable tool for comprehensive protein characterization.
- Addressing technical challenges enhances the utility of TDMS.
- Further advancements in TDMS will improve proteomic analysis.
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