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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Related Experiment Video

Updated: Jul 14, 2026

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
09:15

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Published on: August 16, 2019

[New fixative for the biological material].

S A Araslanov, V B Zaĭtsev, A G Meshandin

    Morfologiia (Saint Petersburg, Russia)
    |May 29, 2007
    PubMed
    Summary

    A new, non-toxic biological fixative using copper cations has been patented. This novel fixative offers selective tissue staining for improved microscopic analysis.

    Area of Science:

    • Biochemistry
    • Histology
    • Materials Science

    Context:

    • Traditional fixatives can be toxic and may obscure fine tissue details.
    • Developing safer and more effective histological preparation methods is crucial for biological research.

    Purpose:

    • To introduce a novel, non-toxic fixative for biological specimens.
    • To optimize the concentration of bivalent copper cations for effective fixation.
    • To evaluate the fixative's utility in conjunction with silver nitrate staining techniques.

    Summary:

    • A new patent describes a non-toxic fixative based on aqueous solutions of bivalent copper cations.
    • The optimal molarity for this copper cation fixative was determined to be 0.2 M.
    • This method demonstrates selective tissue structure visualization, particularly when using Bielschowsky-Gros impregnation with silver nitrate.

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    Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
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    Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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    Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

    Published on: July 13, 2016

    Related Experiment Videos

    Last Updated: Jul 14, 2026

    Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
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    Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

    Published on: August 16, 2019

    Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
    10:21

    Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

    Published on: December 25, 2017

    Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
    07:42

    Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

    Published on: July 13, 2016

    Impact:

    • Provides a safer alternative to toxic histological fixatives.
    • Enhances the selective demonstration of tissue structures for clearer microscopic examination.
    • Facilitates improved diagnostic capabilities and research insights in histology and related fields.