Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatic plasma membrane fluidity and dietary proteins.

V Kumar1, U K Misra

  • 1Department of Biochemistry, University of Delhi.

Indian Journal of Biochemistry & Biophysics
|August 1, 1991
PubMed
Summary

Protein deficiency decreases liver plasma membrane fluidity in rats. Supplementing with lysine and threonine improved membrane fluidity, highlighting the importance of diet for cell membrane health.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corticosteroids in myasthenia gravis: Rethinking oral corticosteroid therapy.

Journal of the neurological sciences·2026
Same author

Specialty grand challenge in neuroinfectious diseases.

Frontiers in neurology·2025
Same author

A Study of Catecholamine Levels in Acute ICH Patients and its Clinical Significance.

The Journal of the Association of Physicians of India·2022
Same author

Safety and Efficacy of Sovateltide (IRL-1620) in a Multicenter Randomized Controlled Clinical Trial in Patients with Acute Cerebral Ischemic Stroke.

CNS drugs·2021
Same author

Complications of tuberculous meningitis and their effect on outcome in a tertiary care cohort.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2020
Same author

Impaired Autophagy Flux is Associated with Proinflammatory Microglia Activation Following Japanese Encephalitis Virus Infection.

Neurochemical research·2020

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nutritional Science

Background:

  • Dietary protein intake significantly impacts cellular functions.
  • Hepatic plasma membrane fluidity is crucial for various cellular processes.
  • Understanding the effects of protein deficiency on membrane properties is essential.

Purpose of the Study:

  • To investigate the impact of a protein-deficient diet on rat hepatic plasma membrane fluidity.
  • To assess the role of specific amino acids (lysine and threonine) in mitigating these effects.
  • To analyze associated changes in membrane composition and enzyme activities.

Main Methods:

  • Utilized steady-state fluorescence polarization and anisotropy to measure membrane fluidity.
  • Analyzed phospholipid and cholesterol content and fatty acid composition.
  • Assessed the turnover of phosphatidyl choline (PC).
  • Measured the activities of key membrane-bound enzymes.

Main Results:

  • Protein deficiency led to decreased hepatic plasma membrane fluidity, indicated by increased fluorescence polarization and anisotropy.
  • Significant alterations observed in phospholipid and cholesterol content, fatty acid unsaturation, and PC turnover.
  • Changes in membrane-bound enzyme activities included decreased 5'-nucleotidase and phosphodiesterase, and increased (Na(+)-K+)ATPase and adenyl cyclase.
  • Dietary supplementation with lysine and threonine partially reversed these detrimental effects.

Conclusions:

  • Protein deficiency adversely affects hepatic plasma membrane fluidity and function in rats.
  • Lysine and threonine supplementation demonstrate a protective role, suggesting their importance in maintaining membrane integrity.
  • Dietary composition plays a critical role in regulating cell membrane fluidity and associated biochemical processes.

Related Experiment Videos