Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 14, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Simultaneous multicolor imaging of five different lymphatic basins using quantum dots.

Hisataka Kobayashi1, Yukihiro Hama, Yoshinori Koyama

  • 1Molecular Imaging Program and Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892-1088, USA. Kobayash@mail.nih.gov

Nano Letters
|May 29, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolotheranostics of pancreatic cancer by targeting choline, glutamine, and glucose transporters with photoimmunotherapy.

Npj imaging·2026
Same author

Human epidermal growth factor receptor 3-targeted near-infrared photoimmunotherapy in a xenograft mouse model of breast cancer.

BMC cancer·2026
Same author

Epidermal growth factor receptor-targeted near-infrared photoimmunotherapy for malignant pleural mesothelioma.

Lung cancer (Amsterdam, Netherlands)·2026
Same author

Understanding tumor microenvironment dynamics and immune checkpoint inhibitor efficacy following mEGFR-targeted near-infrared photoimmunotherapy.

Cancer letters·2026
Same author

Near-infrared Photoimmunotherapy Targeting High-risk Human Neuroblastoma Cells Expressing GD2.

Anticancer research·2025
Same author

Enhancement of drug delivery through fibroblast activation protein-targeted near-infrared photoimmunotherapy.

JCI insight·2025

This study demonstrates multicolor imaging of lymphatic systems using five quantum dots. This technique allows simultaneous visualization of lymphatic fluid flow, aiding in understanding cancer metastasis routes.

Area of Science:

  • Biomedical imaging
  • Nanotechnology
  • Cancer research

Background:

  • Quantum dots offer high fluorescence intensity and nanoscale properties ideal for lymphatic imaging.
  • Current methods may lack the resolution or multiplexing capability for detailed lymphatic flow analysis.

Purpose of the Study:

  • To develop and demonstrate simultaneous multicolor in vivo spectral fluorescence lymphangiography.
  • To visualize multiple lymphatic flows concurrently using spectrally distinct quantum dots.

Main Methods:

  • Utilized five quantum dots with similar physical sizes but different emission spectra.
  • Performed direct interstitial injection for in vivo imaging.
  • Employed wavelength-resolved spectral fluorescence to differentiate signals.

More Related Videos

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Related Experiment Videos

Last Updated: Jul 14, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

Main Results:

  • Achieved simultaneous, noninvasive visualization of five separate lymphatic flows.
  • Demonstrated the capability of spectrally distinct quantum dots for multiplexed lymphangiography.
  • Confirmed the suitability of quantum dots for high-resolution lymphatic imaging.

Conclusions:

  • Multicolor spectral fluorescence lymphangiography using quantum dots is feasible for simultaneous visualization of multiple lymphatic pathways.
  • This technique has potential implications for predicting cancer metastasis routes by mapping lymphatic drainage.
  • Quantum dots provide a powerful tool for advanced in vivo lymphatic system research.