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A scheme for the identification of viridans streptococci
D Beighton1, J M Hardie, R A Whiley
1Hunterian Dental Research Unit, London Hospital Medical College.
Journal of Medical Microbiology
|December 1, 1991
Summary
This study developed an improved biochemical scheme for identifying oral viridans streptococci species. The 14-test method accurately differentiates all recognized species and subtypes within Streptococcus sanguis.
Area of Science:
- Microbiology
- Oral Biology
- Bacteriology
Background:
- Viridans streptococci are common oral bacteria with diverse species.
- Accurate identification of these species is crucial for understanding oral health and disease.
- Current identification schemes for viridans streptococci have limitations.
Purpose of the Study:
- To develop and validate an improved biochemical identification scheme for oral viridans streptococci.
- To differentiate all currently recognized species of viridans streptococci.
- To distinguish biotypes within Streptococcus sanguis.
Main Methods:
- Tested a collection of strains representing all recognized oral viridans streptococci species.
- Assessed glycosidase activities using 4-methylumbelliferyl-linked fluorogenic substrates.
- Performed conventional fermentation and hydrolytic tests.
Main Results:
- A biochemical scheme comprising 14 tests was established.
- The scheme successfully differentiated all tested oral viridans streptococci species.
- Three distinct biotypes within Streptococcus sanguis were identified.
Conclusions:
- The developed 14-test biochemical scheme offers improved accuracy for viridans streptococci identification.
- This scheme enhances the differentiation of oral streptococci species and biotypes.
- The findings represent an advancement over existing identification methods for viridans streptococci.