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Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
Published on: December 16, 2016
Towards hematopoietic reconstitution from embryonic stem cells: a sanguine future
Shannon L McKinney-Freeman1, George Q Daley
1Department of Medicine, Division of Hematology/Oncology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Current Opinion in Hematology
|May 31, 2007
Summary
Hematopoietic stem cells (HSCs) and blood cells can be derived from embryonic stem cells (ESCs). Further research is needed to overcome challenges before clinical applications for HSC transplantation are realized.
Area of Science:
- Stem cell biology
- Hematopoiesis
- Regenerative medicine
Background:
- Embryonic stem cells (ESCs) hold promise for regenerative medicine.
- Hematopoiesis is the process of blood cell formation.
Purpose of the Study:
- Review recent advancements in deriving hematopoietic stem cells (HSCs) and blood lineages from ESCs.
- Identify challenges hindering clinical applications of ESC-derived HSCs.
Main Methods:
- Review of current literature on ESC differentiation protocols.
- Analysis of studies reporting derivation of hematopoietic cells from ESCs.
Main Results:
- Successful derivation of hematopoietic repopulating cells, red blood cells, and T cells from murine and human ESCs.
- Identified need to characterize ESC-derived HSC phenotype and purge teratoma-forming cells.
- Highlighted necessity for optimized in-vivo models for human ESC-derived HSC assessment.
- Noted that ESC progeny often represent primitive, not definitive, adult hematopoietic cells.
Conclusions:
- It is possible to isolate HSCs and mature blood lineages from both mouse and human ESCs.
- ESCs represent a valuable potential source for transplantable HSCs.
- Further research is required to address remaining challenges for clinical translation.
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