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Electrophoresis: Overview

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Capillary Electrophoresis: Applications

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High-Performance Liquid Chromatography: Types of Detectors

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Gas Chromatography: Types of Detectors-II

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Related Experiment Video

Updated: Jul 14, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Multiphoton excitation fluorescence: a versatile detection method for capillary electrophoresis.

Wei Du¹, Sheng Chen, Youzhi Xu

¹Key Laboratory of Biomedical Photonics of MOE, Hubei Bioinformatics, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Journal of Separation Science

|June 1, 2007

Summary

Multiphoton excitation fluorescence (MPEF) offers a novel detection method for capillary electrophoresis (CE) in bioanalysis. This review covers MPEF fundamentals, techniques, and applications, highlighting recent advances in CE-MPEF.

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Published on: September 19, 2018

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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

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Published on: November 21, 2023

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Area of Science:

Quantum Optics
Analytical Chemistry
Biotechnology

Background:

Multiphoton excitation (MPE) is a long-established quantum optics principle.
Multiphoton excitation fluorescence (MPEF) is an emerging technique for bioanalysis.
MPEF is newly applied to microseparation techniques like capillary electrophoresis (CE).

Purpose of the Study:

To review the application of MPEF detection in CE.
To discuss MPEF fundamentals, methods, and detector configurations.
To highlight recent advances and future prospects of CE-MPEF in biological and environmental analyses.

Main Methods:

Review of MPEF principles and excitation approaches.
Analysis of detector configurations for CE-MPEF.
Compilation of applications in biological and environmental fields.
Discussion of recent laboratory advancements in CE-MPEF.

Main Results:

MPEF provides a novel and sensitive detection method for CE.
Various approaches and configurations enable MPEF in CE.
CE-MPEF demonstrates utility in biological and environmental sample analysis.
Recent laboratory work shows promising advancements in CE-MPEF.

Conclusions:

MPEF is a developing yet powerful tool for CE-based bioanalysis.
Further research and development are expected to expand CE-MPEF applications.
CE-MPEF holds significant potential for future analytical challenges.