Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton08:50

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

1.1K
In this work, an in vitro reconstitution approach is employed to study the poroelasticity of actomyosin gels under controlled conditions. The dynamics of the actomyosin gel and the embedded solvent are quantified, through which the network poroelasticity is demonstrated. We also discuss the experimental challenges, common pitfalls, and relevance to cell cytoskeleton...
1.1K
Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix09:13

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

3.5K
This protocol presents a method to perform rheology characterization of mucus that resides on gill rakers (GRs) of the silver carp. Viscoelastic characteristics of GR-mucus, obtained by measuring viscosity, storage and loss moduli, are evaluated for the apparent yield stress to understand the filter feeding mechanism in...
3.5K
The Diffusion of Passive Tracers in Laminar Shear Flow08:01

The Diffusion of Passive Tracers in Laminar Shear Flow

9.0K
A protocol for the study of the diffusion of passive tracers in laminar pressure-driven flow is presented. The procedure is applicable to various capillary pipe...
9.0K
Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions11:51

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

9.1K
Two different methods for characterizing the incipient particle motion of a single bead as a function of the sediment bed geometry from laminar to turbulent flow are...
9.1K
An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids11:03

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

9.0K
An experimentally accessible analog method for studying molecular hydrodynamic processes in dense fluids is presented. The technique uses particle image velocimetry of vibrated, high-restitution grain piles and allows direct, macroscopic observation of dynamical processes known and predicted to exist in strongly interacting, high density gases and...
9.0K
Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

12.5K
Confocal microscopy is used to image quiescent and flowing colloid-polymer mixtures, which are studied as model systems for attractive suspensions. Image analysis algorithms are used to calculate structural and dynamic metrics for the colloidal particles that measure changes due to geometric...
12.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated homocysteine is associated with increased rates of epigenetic aging in a population with mild cognitive impairment.

Aging cell·2024
Same author

Human trials exploring anti-aging medicines.

Cell metabolism·2024
Same author

Nicotinamide riboside and pterostilbene reduces markers of hepatic inflammation in NAFLD: A double-blind, placebo-controlled clinical trial.

Hepatology (Baltimore, Md.)·2022
Same author

Correction: SIRT2 Ablation Has No Effect on Tubulin Acetylation in Brain, Cholesterol Biosynthesis or the Progression of Huntington's Disease Phenotypes In Vivo.

PloS one·2021
Same author

Safety Assessment of High-Purity, Synthetic Nicotinamide Riboside (NR-E) in a 90-Day Repeated Dose Oral Toxicity Study, With a 28-Day Recovery Arm.

International journal of toxicology·2020
Same author

NAD<sup>+</sup> supplementation rejuvenates aged gut adult stem cells.

Aging cell·2019

Related Experiment Video

Updated: Jan 13, 2026

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix
09:13

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

Published on: July 10, 2020

3.5K

Two neurons mediate diet-restriction-induced longevity in C. elegans.

Nicholas A Bishop1, Leonard Guarente

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Nature
|June 1, 2007
PubMed
Summary

Dietary restriction extends lifespan by activating the skn-1 gene in specific neurons. This signals other body tissues to boost metabolism, proving a neuronal link to longevity.

More Related Videos

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions
11:51

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Published on: February 22, 2018

9.1K
An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids
11:03

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

9.0K

Related Experiment Videos

Last Updated: Jan 13, 2026

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix
09:13

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

Published on: July 10, 2020

3.5K
Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions
11:51

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Published on: February 22, 2018

9.1K
An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids
11:03

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

9.0K

Area of Science:

  • Cellular biology
  • Neuroscience
  • Genetics

Background:

  • Dietary restriction (DR) is known to extend lifespan and delay aging across species.
  • DR significantly impacts endocrine function in mammals, but a direct hormonal link to longevity remains unproven.
  • The molecular mechanisms by which DR influences longevity are not fully understood.

Purpose of the Study:

  • To investigate the causal role of hormonal signals in diet-restricted longevity.
  • To identify the specific genes and cellular pathways involved in DR-mediated lifespan extension.
  • To elucidate the communication mechanisms between neurons and peripheral tissues in response to DR.

Main Methods:

  • Utilized the model organism Caenorhabditis elegans for lifespan studies.
  • Focused on the transcription factor gene skn-1 and its role in ASI neurons.
  • Investigated the signaling pathways from neurons to peripheral tissues.

Main Results:

  • Dietary restriction-induced longevity in C. elegans requires the transcription factor gene skn-1.
  • skn-1 acts within the ASI neurons, a pair of sensory neurons in the head.
  • DR activates skn-1 in ASI neurons, which then signal to peripheral tissues to increase metabolic activity.

Conclusions:

  • Increased lifespan in diet-restricted metazoans depends on cell non-autonomous signaling.
  • ASI neurons mediate DR-induced longevity through an endocrine mechanism involving skn-1.
  • This study demonstrates a causal role for a specific neuronal signaling pathway in DR longevity.