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Micro-bioreactor array for controlling cellular microenvironments.

Elisa Figallo1, Christopher Cannizzaro, Sharon Gerecht

  • 1Department of Chemical Engineering, University of Padova, Italy.

Lab on a Chip
|June 1, 2007
PubMed
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A novel micro-bioreactor array (MBA) enables high-throughput studies of cell differentiation. This system allows controlled investigation of factors like hydrodynamic shear on cell behavior, particularly for human embryonic stem cells (hESCs).

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Investigating cell differentiation requires high-throughput methods to analyze multiple regulatory factors.
  • Existing methods may lack the control and throughput needed for complex cellular studies.

Purpose of the Study:

  • To develop and validate a micro-bioreactor array (MBA) for controlled, high-throughput cell differentiation studies.
  • To enable spatial and temporal analysis of factors influencing cell differentiation, including hydrodynamic shear.

Main Methods:

  • Fabrication of a 12-bioreactor array using soft lithography.
  • Cultivation of cells (adherent or hydrogel-encapsulated) with variable hydrodynamic shear.
  • Computational fluid dynamic (CFD) modeling for flow and mass transport characterization.

Related Experiment Videos

  • Automated image analysis of cell differentiation markers.
  • Validation with C2C12 cells, primary rat cardiac myocytes, and human embryonic stem cells (hESCs).

    • Main Results:

    • The micro-bioreactor array (MBA) successfully supported cell cultivation under varied conditions.
    • Computational fluid dynamic (CFD) modeling provided insights into flow dynamics within the MBA.
    • Correlations between smooth muscle actin expression and cell density were established for hESCs under different flow configurations.

    Conclusions:

    • The developed micro-bioreactor array (MBA) is a versatile platform for high-throughput cell differentiation research.
    • The MBA facilitates controlled studies on stem cell differentiation, offering insights into the impact of microenvironment factors.
    • This technology supports advanced investigations into stem cell behavior and differentiation pathways.