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[Study on HPEC fingerprint of cultured Cordyceps militaris]
Bin Ye1, Li-yan Song, Rong-min Yu
1College of Pharmacy, Jinan University, Guangzhou 510632, China.
Objective:
To investigate the High Performance Capillary Electrophoresis (HPCE) fingerprint of cultured Cordyceps militaris (L.) Link.
Methods:
Separation was performed on a 50 cm x 75 microm uncoated capillary with 0.5 mmol/L borate solution (pH 9. 18) as HPEC buffer. The run voltage was 20 Kv, temperature 25 degrees C and the DAD detection was set at 254nm.
Results:
Fingerprint consisted of 11 common peaks. The validation of methods was satisfied with the requirements for SFDA's technical regulations.
Conclusion:
The method was accurate and simple and suitable to the quality control of cultured Cordyceps militaris (L.) Link.