Related Experiment Video
Updated: Jul 14, 2026
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 27, 2014
[A new method in determination of formononetin content in Trifolium pratense]
Guang-wei Zhou1, Hui Mu, Yue-feng Li
1Department of Chemistry, Xián Jiaotong University, Xián 710049, China.
Objective:
A new method was established to determine the formononetin content in Trifoblium pratense L.
Methods:
Effects of fluorescence intensity, as well as its precision, repetition and recovery efficency were studied.
Results:
The linear relationship was y = 1440 x -0.263, r = 0.999, the detection limit was 1.50 x 10(-9) mol/L, the relative deviation between this method and HPLC method was 1.65%.
Conclusion:
The study indicated that this method was viable, and could be used in both scientific research and industrial production.
