Related Experiment Video

Updated: Jul 14, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

Matrix metalloproteinase-3 (stromelysin-1) in acute inflammatory tissue injury

Kamalakar C Nerusu1, Roscoe L Warner, Narasimharao Bhagavathula

  • 1Department of Pathology, The University of Michigan Medical School, 1301 Catherine Road/Box 0602, Ann Arbor, MI 48109, USA.

Experimental and Molecular Pathology
|June 2, 2007
PubMed

Insights

Mice lacking matrix metalloproteinase-3 (MMP-3) showed reduced tissue injury in lung and peritoneum. MMP-3 plays a key role in acute inflammatory responses and tissue damage.

Area of Science:

  • Immunology
  • Molecular Biology
  • Pathology

Background:

  • Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1, is an enzyme involved in tissue remodeling.
  • The role of MMP-3 in acute inflammatory tissue injury is not fully understood.

Purpose of the Study:

  • To investigate the role of MMP-3 in acute inflammatory tissue injury using a mouse model.
  • To determine if MMP-3 deficiency impacts injury resulting from immune complex formation or cytokine instillation.

Main Methods:

  • Mice lacking MMP-3 and wild-type control mice were used.
  • Immune complexes were formed in the alveolar or peritoneal walls.
  • Intra-tracheal instillation of macrophage inhibitory protein-2 (MIP-2) was performed.
  • Tissue injury was assessed histologically.
  • Accumulation of anti-laminin 111 and neutrophil influx into lavage fluid were quantified.

Main Results:

  • Mice lacking MMP-3 exhibited significantly less tissue injury compared to normal mice after immune complex formation.
  • MMP-3 deficient mice showed reduced lung injury following MIP-2 instillation.
  • Tissue injury correlated with the accumulation of anti-laminin 111 material and neutrophil influx in bronchoalveolar lavage (BAL) or peritoneal lavage (PL) fluid.

Conclusions:

  • MMP-3 plays a significant role in mediating acute inflammatory tissue injury.
  • Targeting MMP-3 may be a potential therapeutic strategy for inflammatory conditions.

More Related Videos

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels07:39

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Published on: January 22, 2019

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Acute Inflammation III: Local and Systemic Effects01:25

Acute Inflammation III: Local and Systemic Effects

Acute inflammation produces a coordinated set of local and systemic changes that limit injury, eliminate pathogens, and initiate repair. These responses arise within minutes of infection, trauma, or chemical insult and are driven by vascular alterations and leukocyte-derived mediators. When the stimulus resolves, the reaction typically abates within days.Local EffectsAt the site of injury, arteriolar vasodilation increases blood flow, resulting in redness and warmth. Simultaneously, increased...
Acute Inflammation I: Inflammatory Response01:26

Acute Inflammation I: Inflammatory Response

Acute inflammation is a rapid, short-lived physiological response to tissue injury or infection, designed to eliminate harmful agents and initiate repair. This tightly regulated process typically lasts from minutes to several days and is triggered by factors such as microbial invasion, physical trauma, or chemical injury.Recognition and Mediator ReleaseThe inflammatory response begins when resident immune cells—such as mast cells, macrophages, and dendritic cells—detect damage-associated...
Acute Inflammation II: Cellular Phase01:26

Acute Inflammation II: Cellular Phase

The cellular phase of acute inflammation is a tightly orchestrated sequence of events that recruits leukocytes, primarily neutrophils, to sites of tissue injury or infection. Following the initial vascular changes, this phase ensures effective immune cell migration, activation, and function at the affected site to eliminate pathogens and initiate tissue repair.Leukocyte Recruitment CascadeLeukocyte recruitment happens in four steps: margination, adhesion, transmigration, and chemotaxis. Reduced...
Inflammation01:38

Inflammation

Overview
Chronic Inflammation: Introduction01:12

Chronic Inflammation: Introduction

Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...