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Updated: Jul 14, 2026
Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
Two new sesquiterpenes from Alisma orientalis.
Zhi-Yong Jiang1, Xue-Mei Zhang, Jun Zhou
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan, PR China.
Two new compounds from Alisma orientalis, alismorientols A and B, were identified. Alismorientol A demonstrated moderate activity against Hepatitis B virus (HBV) in laboratory tests.
Area of Science:
- Natural Product Chemistry
- Medicinal Chemistry
- Virology
Background:
- Alisma orientalis is a traditional medicinal plant.
- Sesquiterpenoids are a class of natural products with diverse biological activities.
- Hepatitis B virus (HBV) infection remains a significant global health concern.
Purpose of the Study:
- To isolate and characterize new compounds from Alisma orientalis.
- To evaluate the anti-HBV activity of the isolated compounds.
Main Methods:
- Isolation of compounds using chromatographic techniques.
- Structure elucidation using 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and X-ray crystallography.
- In vitro anti-HBV bioassays to determine IC50 values for HBsAg and HBeAg.
Main Results:
- Two new sesquiterpenoids, alismorientols A (1) and B (2), were successfully isolated and identified.
- Compound 1 exhibited moderate in vitro activity against HBV, with IC50 values of 1.1 µM for HBsAg and 14.7 µM for HBeAg.
- Compound 2's activity was not specified in the abstract.
Conclusions:
- Alisma orientalis is a source of novel sesquiterpenoids.
- Alismorientol A shows potential as an anti-HBV agent, warranting further investigation.
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