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Related Experiment Videos

Homocysteine modulates the CD40/CD40L system.

Cesaria Prontera1, Nicola Martelli, Virgilio Evangelista

  • 1Department of Medicine, Gabriele d'Annunzio University Foundation, Chieti-Pescara, Italy.

Journal of the American College of Cardiology
|June 5, 2007
PubMed
Summary
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Elevated homocysteine levels significantly increase soluble CD40L and CD40 expression, suggesting a link between hyperhomocysteinemia and cardiovascular disease risk.

Area of Science:

  • Cardiovascular Research
  • Molecular Biology
  • Immunology

Background:

  • Hyperhomocysteinemia (HHcy) is linked to increased atherothrombosis.
  • The CD40/CD40 ligand (CD40L) dyad plays a role in vascular inflammation and atherosclerosis pathogenesis.

Purpose of the Study:

  • To investigate the impact of HHcy on the CD40/CD40L dyad.
  • To explore the molecular mechanisms connecting HHcy to cardiovascular disease.

Main Methods:

  • Measured soluble CD40L (sCD40L) levels in HHcy patients and healthy controls using ELISA.
  • Assessed CD40 and VCAM-1 expression in endothelial cells and CD40L in platelets using real-time PCR and flow cytometry after homocysteine exposure.

Main Results:

  • HHcy patients exhibited significantly higher sCD40L levels.

Related Experiment Videos

  • Homocysteine exposure increased CD40 expression in endothelial cells and CD40L expression in platelets.
  • Homocysteine enhanced CD40L-induced VCAM-1 expression in endothelial cells.

    • Conclusions:

    • The CD40/CD40L dyad is a potential molecular target of homocysteine.
    • Upregulation of CD40/CD40L signaling may mediate the increased cardiovascular disease risk associated with HHcy.