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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
Monomeric red fluorescent protein as a reporter for macromolecular localization in Streptomyces coelicolor
Khoa Duy Nguyen1, Stephanie H Au-Young, Justin R Nodwell
1Department of Biochemistry and Biomedical Sciences, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ont., Canada L8N 3Z5.
Abstract:
The enhanced green fluorescent protein (eGFP) is widely used to investigate cell type specific gene expression and protein localization in the filamentous streptomycetes. To broaden the scope of cell biological investigation in these organisms, we have adapted shuttle vectors for the construction of gene fusions to the monomeric red fluorescent protein (mRFP1) and have tested them in Streptomyces coelicolor. Using fusions of mRFP1 to the cell division proteins DivIVA and FtsZ, we show that mRFP1 is comparable to eGFP for cell biological research in this organism and suggest that this paves the way for the future use of two-color imaging and FRET.
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