Balance module allows consistent monitoring and documentation of the pooling process for nucleic acid amplification...

When performing minipool testing of blood donations for transfusion-transmissible viruses, it is crucial to correctly dispense plasma from each donation into the pools. However, concerns regarding the monitoring and documentation of the pooling process exist.

A balance module with a tube holder has been developed, which can be easily integrated into liquid handling platforms. The existing software monitors and evaluates every single dispensing from primary samples to the minipool to confirm liquid arrival. The weighing accuracy of the balance module is approximately 2 mg per dispensing episode.

Ten thousand one hundred and fifty-six blood donations were tested on a Tecan Genesis RSP pipetting system. Thirty-one donations were not pipetted because the pipetting workstation identified a clot or sample shortage in the primary tube. The balance module exclusively detected another 18 mispipettings, which were outside of the acceptance criteria of 100 +/- 10%. The mean pipetted volume for these samples was 34.2 microl (range 0-87 microl). Visual inspection of the corresponding primary tubes showed blood clots, short sample or no apparent cause. The average deceleration of the pooling process, using the balance, was determined to be about 22%.

With the novel liquid arrival check system, complete and consistent process documentation of pooling for nucleic acid amplification technology (NAT) testing is feasible. It enables blood banks to monitor and compare every single dispense made with predefined, required volumes. Results can be transferred to the laboratory information management system for automated selective exclusion of inaccurately dispensed samples. ONE SENTENCE SUMMARY: Monitoring and documenting the pooling process for NAT testing using a balance-based liquid arrival check system.