Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular diversity in engineered protein libraries.

Nora H Barakat1, John J Love

  • 1Department of Chemistry and Biochemistry, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-1030, USA.

Current Opinion in Chemical Biology
|June 6, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Incorporating Metal-Ligand and Salt-Bridge Interactions in the Design of Protein Heterodimers.

Biochemistry·2025
Same author

Design of High-Affinity Metal-Controlled Protein Dimers.

Biochemistry·2019
Same author

Characterizing substrate selectivity of ubiquitin C-terminal hydrolase-L3 using engineered α-linked ubiquitin substrates.

Biochemistry·2014
Same author

DNA fingerprint analysis of three short tandem repeat (STR) loci for biochemistry and forensic science laboratory courses.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2011
Same author

Combined use of experimental and computational screens to characterize protein stability.

Protein engineering, design & selection : PEDS·2010
Same author

Chemical synthesis of two series of nerve agent model compounds and their stereoselective interaction with human acetylcholinesterase and human butyrylcholinesterase.

Chemical research in toxicology·2009
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

Engineered protein libraries create diverse mutant protein variants for novel properties. Combining computational methods with screening accelerates protein design and discovery.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Engineered protein libraries are collections of mutant protein variants.
  • These libraries are designed for high molecular diversity, ranging from hundreds to over 10^12 members.
  • The primary goal is to discover new protein variants with desired novel properties through screening.

Purpose of the Study:

  • To summarize the design and application of engineered protein libraries.
  • To highlight strategies for generating molecular diversity in protein engineering.
  • To discuss advancements in protein design methodologies.

Main Methods:

  • Utilizing large-scale mutant protein variant collections (engineered protein libraries).
  • Employing screening methods to identify variants with specific properties.

Related Experiment Videos

  • Leveraging the genetic code for 'focused' library design biased towards favorable biophysical properties.
  • Integrating computational algorithms with in vivo screening techniques.

    • Main Results:

    • Engineered protein libraries enable the generation of vast molecular diversity.
    • Focused libraries offer biased selection for desirable biophysical characteristics.
    • The combination of computational approaches and in vivo screening enhances success rates in protein design.

    Conclusions:

    • Engineered protein libraries are crucial tools for discovering novel protein functions.
    • Strategic library design, including focused approaches, optimizes the search for specific protein properties.
    • Emerging computational and in vivo screening methods are revolutionizing protein design.