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Single molecule fluorescence spectroscopy of pH sensitive oligonucleotide switches

Branko Kolaric1, Michel Sliwa, Marco Brucale

  • 1Katholieke Universiteit Leuven, Department Chemistry and Institute for Nanoscale Physics and Chemistry (INPAC), Celestijnenlaan 200F, Heverlee, B-3001, Belgium.

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|June 6, 2007
PubMed
Summary

Oligonucleotide constructs act as pH-sensitive switches, opening and closing based on acidity. Single molecule studies reveal complete switching, unlike bulk observations, suggesting DNA construct association at higher concentrations.

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