Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assay for rapid quantification of capped and tailed intact mRNA.

Vaccine·2025
Same author

Analytical Performance of a Multiplexed Microarray Assay for Rapid Identification and Quantification of a Multivalent mRNA Vaccine.

Vaccines·2024
Same author

30-Minute Highly Multiplexed VaxArray Immunoassay for Pneumococcal Vaccine Antigen Characterization.

Vaccines·2022
Same author

Rapid Identity and Quantity CQA Test for Multivalent mRNA Drug Product Formulations.

Vaccines·2022
Same author

Development and Evaluation of a Paper-Based Microfluidic Device for Detection of <i>Listeria monocytogenes</i> on Food Contact and Non-Food Contact Surfaces.

Foods (Basel, Switzerland)·2022
Same author

VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen.

Journal of immunological methods·2022

Related Experiment Video

Updated: Jul 14, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Vapor deposition method for sensitivity studies on engineered surface-enhanced Raman scattering-active substrates.

Thomas H Reilly1, Jordan D Corbman, Kathy L Rowlen

  • 1Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA.

Analytical Chemistry
|June 7, 2007
PubMed
Summary

A new vapor-phase deposition method enhances limit of detection (LOD) studies for engineered surface-enhanced Raman scattering (SERS)-active substrates. This technique achieves highly sensitive analyte deposition, enabling precise LOD determination on small SERS platforms.

More Related Videos

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications
06:15

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

Published on: June 16, 2023

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
03:33

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Related Experiment Videos

Last Updated: Jul 14, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications
06:15

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

Published on: June 16, 2023

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection
03:33

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Quantitative analysis of small, lithographically produced surface-enhanced Raman scattering (SERS)-active substrates presents challenges.
  • Existing methods struggle with precise analyte deposition on engineered SERS platforms.
  • Developing reliable methods for limit of detection (LOD) studies is crucial for SERS applications.

Purpose of the Study:

  • To design and optimize a vapor-phase analyte deposition method for LOD studies.
  • To overcome limitations in quantitative analysis of small SERS substrates.
  • To enable accurate LOD determination on engineered SERS-active substrates.

Main Methods:

  • A custom-built flow cell was utilized for vapor-phase deposition of benzenethiol.
  • Deposition was performed on SERS-active silver (Ag) thin films and nanoaperture arrays fabricated by electron-beam lithography.
  • Surface coverage was monitored using the 1070-cm(-1) band, fitted to Langmuir adsorption kinetics.

Main Results:

  • The vapor deposition method demonstrated reliability, with LOD values consistent with prior reports on control substrates.
  • Application to a 20 x 20 µm² nanoaperture array yielded a low LOD of 4.2 ± 0.3 amol.
  • Langmuir adsorption kinetics accurately described benzenethiol surface coverage over time.

Conclusions:

  • The developed vapor-phase deposition method is effective for quantitative analysis and LOD studies on engineered SERS substrates.
  • This technique significantly improves sensitivity for detecting analytes on small SERS platforms.
  • The method provides a reliable approach for characterizing the performance of novel SERS substrates.