Related Experiment Video
Updated: Jul 14, 2026
06:58
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
Bacteroides coprophilus sp. nov., isolated from human faeces
Hidenori Hayashi1, Kensaku Shibata2,1, Mohammad Abdul Bakir1
1Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Summary
Researchers discovered a new bacterial species, Bacteroides coprophilus, from human feces. This anaerobic Gram-negative bacterium expands our understanding of gut microbiota composition and function.
Area of Science:
- Microbiology
- Gut Microbiome Research
- Bacteriology
Background:
- The human gut harbors a complex microbial ecosystem crucial for health.
- Accurate identification and classification of gut bacteria are essential for understanding their roles.
Purpose of the Study:
- To isolate and characterize novel bacterial species from human fecal samples.
- To determine the phylogenetic and phenotypic properties of newly identified bacteria.
Main Methods:
- Bacterial isolation from human feces.
- Phylogenetic analysis using 16S rRNA gene sequencing.
- Phenotypic characterization including fatty acid analysis and G+C content determination.
Main Results:
- Three novel Gram-negative, anaerobic, rod-shaped bacteria (strains CB40, CB41, CB42(T)) were isolated.
- Phylogenetic analysis placed these strains within the genus Bacteroides, representing a new species.
- Strain CB42(T) showed 93.4% 16S rRNA gene sequence similarity to Bacteroides barnesiae.
Conclusions:
- A novel species, Bacteroides coprophilus sp. nov., is proposed based on distinct phylogenetic and phenotypic characteristics.
- The proposed type strain is CB42(T) (=JCM 13818(T)=DSM 18228(T)).
- This discovery contributes to the catalog of human gut bacteria and their diversity.
Related Concept Videos
01:26Bacterial Phylum Bacteroidota
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
01:27Bacterial Phylum Firmicutes
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
01:30Bacterial Phylum Spirochaetes
Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased by a...
01:27Microbiota of the Large Intestine
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
01:29Bacterial Flora of the Large Intestine
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
01:30Bacterial Phylum Actinobacteria
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...